JMJD1B (KDM3B), FLAG-tag Recombinant

Catalog #

50421

$465 *

Size: 20 µg

Qty

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Description

Recombinant human JMJD1B (KDM3B: lysine demethylase 3B), full length encompassing amino acids 2-1761 (end). The construct contains an N-terminal FLAG-tag followed by an Avi-Tag™. The protein was affinity purified.

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Synonyms

KDM3B, JHDM2B, KIA1082, Jumonji domain-containing protein 1B, JmjC domain-containing histone demethylation protein 2B, nuclear protein 5qNCA, DIJOS, NET22, C5orf7

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

JMJD1B (KDM3B) inhibition curve using an AlphaLISA assay (#79839). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

JMJD1B (KDM3B) (FLAG-Avi-2-1761(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 μg/ml FLAG peptide

195 kDa

Amino Acids

2-1761(end)

Specific Activity

0.02 pmole/min/μg

Genbank #

NM_016604

UniProt #

Q7LBC6

Tag(s)

N-terminal Avi-, FLAG-tags

References

1. Kim, J-Y, et al., Mol. Cel. Biol. 2012. 32(14):2917-2933.
2. Mikhaleva, I.I., et al., Peptides. 2011. 32(4): 826-831.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was performed according to the JMJD1B Homogeneous Assay Kit (BPS Bioscience #79839) with JMJD1B enzyme titrated from 400 ng/rxn – 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Demethylase

Regulatory Status

Research Use Only