JARID1C(KDM5C), FLAG-tag Recombinant

Catalog #

50112

$475 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, GenBank Accession No. NM_004187, full length, a.a. 2-1560(end), with N-terminal FLAG- and His-tags, MW= 181 kDa, expressed in Sf9 cells via a baculovirus expression system.

This product has been cited 4 times.

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Synonyms

SMCX, Smcy homolog, X-linked, KDM5C, XE169

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

JARID1C (KDM5C) inhibition curve using an AlphaLISA assay (#50511-2). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations4

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide

181 kDa

Amino Acids

2–1560(end)

Specific Activity

0.14 pmole/min/μg

Genbank #

NM_004187

UniProt #

P41229

Tag(s)

N-terminal FLAG-tag

References

1. Xu, J., et al. (2008). PLoS One 3(7):e2553
2. Iwase, S., et al. (2007). Cell 128(6):1077-88.
3. Jenson, L.R., et al. (2005). Am. J. Hum. Genet. 76(2):227-36.

Storage/Stability

>6 months at –80°C.

Assay Conditions

10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1C (50 – 200 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Demethylase

Regulatory Status

Research Use Only