JARID1A (KDM5A) Homogeneous Assay Kit

Catalog #: 50510
Size: 384 reactions
JARID1A, also known as RBBP2 and KDM5A, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3.
The JARID1A Homogeneous Assay Kit is designed to measure activity of the JARID1A for screening and profiling applications, using AlphaLISA® technology. The key to the JARID1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Supplied As:
The JARID1A Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1A for 384 enzyme reactions.
Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.


Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
>6 months at –80°C
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
1. DiTacchio L, Le HD, Vollmers C et al. Science 2011; 333(6051):1881-5.

The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request.

Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Assay Kit
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