ISRE Reporter-HEK293 Recombinant Cell Line (JAK pathway)
The ISRE Reporter – HEK293 Cell Line is designed to monitor the activity of the JAK/STAT signaling pathway. The JAK (Janus kinase) /STAT (Signal Transducer and Activator of Transcription) pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins.
Binding of Interferon alpha (IFNα) to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylates and activates STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex known as ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region thereby promoting transcription of interferon-inducible genes.
The ISRE Reporter – HEK293 Cell Line contains the firefly luciferase gene under the control of ISRE stably integrated into HEK293 cells. This cell line is validated for the response to stimulation with interferon Alpha and to treatment with JAK inhibitor Pyridone 6.
Interested in screening and profiling inhibitors, blocking antibodies, or activators of the JAK pathway without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
This product has been cited 2 times.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Materials Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 1 | BPS Bioscience #60187 |
| Growth Medium 1B | BPS Bioscience #79531 |
Materials Required for Cellular Assay
| Name | Ordering Information |
| Human Interferon Alpha A (IFNα) | R&D Systems #11100-1/PBL Assay Service #11100-1 |
| JAK Inhibitor I (Pyridone 6): Prepare stock solution in DMSO. | EMD Millipore # 420099 |
| Assay Medium: Thaw Medium 1 | BPS Bioscience #60187 |
| Growth Medium 1B | BPS Bioscience #79531 |
| 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate | |
| ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
| Luminometer |
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1B (BPS Bioscience #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin
Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)
The cell line has been screened to confirm the absence of Mycoplasma species.
1. Hebenstreit D et al. (2005) JAK/STAT-dependent gene regulation by cytokines. Drug News Perspect 18(4): 243–249.
2. Pedranzini L et al. (2006) Pyridone 6, a pan-Janus-activated kinase inhibitor, induces growth inhibition of multiple myeloma cells. Cancer Res. 66(19):9714-9721.
Application Reference(s):
1. Broussonetia papyrifera Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples (2020)
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