JAK1 (Janus Kinase 1) Assay Kit

Catalog #

79518

$560 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Janus kinase (JAK) is a family of intracellular, nonreceptor tyrosine kinases that play an important role in cytokine-mediated signaling. This family includes four protein kinases; JAK1, JAK2, JAK3 and Tyk2. Various studies have suggested that regulation of JAK activity should be a promising approach to combat numerous diseases such as immune deficiencies and cancers. The JAK1 (Janus Kinase 1) Assay Kit is designed to measure JAK1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against JAK1? Check out our Kinase Screening Services.

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Synonyms

Tyrosine-protein kinase JAK1, JAK-1, JAK1A, JAK1B

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The JAK1 (Janus Kinase 1) Assay Kit comes in a convenient 96-well format, with enough purified recombinant JAK1 enzyme, JAK1 substrate peptide (IRS-1tide), ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40449	JAK1	10 µg	-80°C	Avoid multiple freeze/thaw cycles!
79334	5x Kinase assay buffer 1	1.5 ml	-20°C
79686	ATP (500 μM)	100 µl	-20°C
79519	10x IRS1-tide	500 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P23458

References

Alicea, N.L. et al., Current Drug Targets 12(4):546-555 (2011)

Storage/Stability

Up to 6 months when stored as recommended.

Instructions for Use

See assay kit data sheet for detailed protocol

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only