ISRE Reporter Kit (JAK/STAT Signaling Pathway)

Catalog #: 60613
Size: 500 reactions
$550
Background:
The JAK (Janus kinase) /STAT (Signal Transducer and Activator of Transcription) pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins. Interferon alpha (IFNa) is a Type I interferon. Binding of IFNa to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region thereby promoting transcription of interferon-inducible genes.
Description:
The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
Synonym(s):
JAK reporter, STAT reporter, ISRE reporter, IFN, Cell based assay kit, cell-based assay, reporter assay kit
Supplied As:
Reporter: ISRE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
Concentration:

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

Format:
assay kit
Instructions for use:
See data sheet insert for detailed transfection and assay protocols.
Storage / Stability:

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Application(s):
• Monitor IFNa-induced JAK/STAT signaling pathway activity.
• Screen for activators or inhibitors of the JAK/STAT signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.
Notes:

The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.

Warning(s):
Avoid freeze/thaw cycles
Product Type:
Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60613.pdf