ISRE Reporter Kit (JAK/STAT Signaling Pathway)
The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
For assays in more difficult to transfect cell types, we suggest using our lentiviral products. The corresponding lentiviral product for this kit is BPS Bioscience #79824.
This product has been cited 2 times.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)