IRAK4, GST-tag Recombinant

Catalog #

40064

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human interleukin-1 receptor-associated kinase 4 (IRAK4), GenBank Accession No. NM_001114182, a.a 2-460(end), with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 78 kDa.

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Synonyms

IRAK4, interleukin-1 receptor-associated kinase 4, NY-REN-64, REN64, IRAK-4

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥67%

Supplied As

Aqueous buffer solution.

Formulation

45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.4 mM KCl, 3 mM DTT, 0.04% Tween-20 and 10% Glycerol.

78 kDa

Amino Acids

2-460(end)

Specific Activity

≥60 pmol/min/µg

Genbank #

NM_001114182

UniProt #

Q9NWZ3

Tag(s)

N-terminal GST-tag

References

1. Ku C.L. et al., J. Exp. Med. 204 (10): 2407- 2422 (2007).
2. Hoarau C. et al., J. Immunol. 179 (7): 4754-4765 (2007).

Application References:

1. Design and synthesis of Imidazo[1,2-b]pyridazine IRAK4 inhibitors for the treatment of mutant MYD88 L265P diffuse large B-cell lymphoma

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Used 0.1 mg/ml MBP as a substrate and 20 μM ATP. Reaction was done for 45 min at 30°C, and the amount of ATP was quantified using Kinase-Glo (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40064 Lot 171027-G

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