Cyclopamine, Free Base
Catalog #: 27013

Size: 5 mg

Structure:

CAS Registry #: 4449-51-8

Purity: >99%

Chemical Formula: C₂₇H₄₁NO₂

Molecular Weight: 411.62

Description: Cyclopamine inhibits activation of the Hedgehog response pathway by binding to
and inhibiting the proto-oncogene Smoothened (SMO). The effects of oncogenic mutations that
either activate SMO or inactivate the tumor suppressor Patched (Ptch) can be reversed by
cyclopamine treatment. Cyclopamine has also been shown to induce apoptosis in colorectal
adenoma cell lines, carcinoma-derived cell lines, and skin basal cell carcinomas and is being
investigated as an anti-cancer drug.

Appearance: White to off-white crystalline solid

Solubility: Soluble in ethanol, methanol and DMSO. Ethanol is the best solvent for biological
use. To make a 2% solution, heat cyclopamine in ethanol to 50-60°C. Store at -20°C. In
methanol or DMSO, solubility is 7 mg/ml and 4 mg/ml respectively.

Biological Activity: Cyclopamine inhibits the activity for Hedgehog signaling-dependent motor
neuron differentiation with IC50 = 300 nmol/L.

Storage/Stability: Store at or below -20°C.

Quality Control: The purity was determined by HPLC analysis.

References:
1. Taipale, J., et al., Nature 2000; 406: 944 – 945.
2. Chen, J.K., et al., Genes & Dev. 2002; 16: 2743 – 2748.
3. Qualtrough, D., et al., Int J Cancer. 2004; 110: 831-837.
4. Tabs, S., and Avci, O. Eur J Dermatol. 2004; 14: 96-102.
5. Fan, Q., et al., Chin J Cancer 2011 Jul; 30(7): 472 – 481.

Click here to view and download product data sheet (PDF)