c-Mer, GST-tag (Human)

Catalog #: 40254
Size: 10 µg
$375
Species: Human
Host Species: Sf9 insect cells
MW: 60 kDa
Genbank #: NM_006343
Tag(s): N-terminal GST-tag
a.a: 578–872
Background:
c-MER is a tyrosine kinase and a member of the MER/AXL/TYRO3 receptor kinase family (1). The c-MER gene encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain. Functional knockout of c-MER in mice shows the mice to have macrophages deficient in the clearance of apoptotic thymocytes thereby demonstrating the critical role for c-MER in the engulfment and efficient clearance of apoptotic cells. Defects in c-MER have been associated with retinitis pigmentosa, a phenotype that can be reversed by replacement of the c-MER gene product (2).
Description:
Human c-Mer, also known as tyrosine-protein kinase Mer, MERTK, and receptor tyrosine kinase MerTK GenBank Accession NM_006343, a.a. 578-872 with N-terminal GST-tag. Expressed in a baculovirus-infected Sf9 cell expression system. MW = 60 kDa.
UniProt
Q12866
Synonym(s):
tyrosine-protein kinase Mer, MERTK, receptor tyrosine kinase MerTK
Specific Activity:
≥50 pmol/min/µg
Assay Conditions:
Assay was done in kinase buffer containing 1 mM DTT using Poly- (Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 1.6 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:

At least 6 months at –80°C.

Application(s):
Useful for the study of enzyme kinetics and for inhibitor screening.
Reference(s):
1. Van Hoof, C. et al., Genomics. 1995; 28: 261-272.
2. Chao, Y. et al., Molec. Cell. 2006; 23: 535-546.
Warning(s):
Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Kinase/Receptor Tyrosine
Product Type:
Recombinant Protein
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Product Datasheets

40254_lot_150929-G.pdf