CTLA4 / IL-2 Reporter - Jurkat Recombinant Cell Line
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of IL-2 promoter with constitutive expression of human CTLA4 (Cytotoxic T-Lymphocyte Associated Protein, CD152; GenBank Accession #NM_005214).
Interested in screening and profiling inhibitors, blocking antibodies, or activators of CTLA4 without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
• Raji cell (ATCC # CCL-86)
• Thaw Medium 2 (BPS Bioscience #60184)
• Growth Medium 2A (BPS Bioscience #60190)
• Assay medium: RPMI1640 medium (Life Technologies #A10491-01)
• Anti-CD3 agonist antibody (BPS Bioscience #71274)
• Anti-CTLA4 neutralizing antibody (Biolegend #349904, clone#L3D10)
• 96-well tissue culture-treated white clear-bottom assay plate
• One-Step luciferase assay system (BPS bioscience # 60690) or other luciferase reagents for measuring firefly luciferase activity
• Luminometer
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.