PD-L1 (Woodchuck) / TCR activator - CHO Recombinant Cell line
Catalog #
79457
$9,400
*
●
●
Purchase
Description
Recombinant CHO-K1 cells constitutively expressing woodchuck (groundhog, Marmota monax) PD-L1 (Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7- H1), GenBank accession # HQ403651) and an engineered T cell receptor (TCR) activator.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
●
Synonyms
Groundhog, Marmota Monax, PD-L1, Programmed Cell Death 1 Ligand 1, CD274, B7 Homolog 1, B7-H1, TCR
●
Product Data Gallery
Product Info
Storage and Usage
Citations
Host Cell Line
CHO
Supplied As
Each vial contains ~2.5 x 10^6 cells in 1 ml of 10% DMSO.
Materials Required But Not Supplied
- • Woodchuck PD-1/ NFAT reporter-Jurkat cell line (BPS Bioscience #79456)
- • Assay Medium: Thaw Medium 2 (BPS Bioscience, #60184)
- • Thaw Medium 3 (BPS Bioscience #60186)
- • Growth Medium 3A (BPS Bioscience #60188)
- • 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
- • ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690)
- • Luminometer
- • Anti-woodchuck PD-1 or PD-L1 neutralizing antibodies (Fisher Scientific #50-146-65, clone #MIH5)
Mycoplasma Testing
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich,#MP0025) to confirm the absence of Mycoplasma species.
Background
The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.