TIGIT - HEK293 Recombinant Cell Line

Catalog #

79332

$4,400 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant HEK293 stably expressing human TIGIT (T cell immunoreceptor with Ig and ITIM domains; VSTM3; VSIG9), GenBank Accession #NM_173799.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Immunotherapy, T cell immunoreceptor with Ig and ITIM domains, HEK293

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Product Data Gallery

Flow cytometry analysis of cell surface expression of TIGIT in TIGIT HEK293 cells

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293 cells

Supplied As

Each vial contains ~3 X 10⁶ cells in 1 ml of 10% DMSO.

Materials Required But Not Supplied

Thaw Medium 1 (BPS Cat. #60187)
Growth Medium 1F (BPS Cat. #79540)
Anti-TIGIT Neutralizing Antibody, PE-Labeled (BPS Cat. #71228)

Genbank #

NM_173799

UniProt #

Q495A1

Mycoplasma Testing

The cell line has been screened using the metabolite-based Mycoplasma Detection Kit (Biotool, #B3903) to confirm the absence of Mycoplasma species.

Background

TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory T cells. Interaction with the poliovirus receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits Src homology (SH) domain-containing protein tyrosine phosphatase SHP1 and SHP2 or the inositol phosphatase SHIP1 and SHIP2 to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-kB and NFAT T cell receptor (TCR) signaling, which blocks T cell proliferation and cytokine production. It serves as a competitive inhibitor of CD226, a co-stimulatory receptor for CD155. TIGIT targeting antibodies that block this T cell-intrinsic inhibitory effects have shown enhanced anti-tumor and anti-viral functions in preclinical studies.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS, #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1F (BPS, #79540): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 100 µg/ml Hygromycin B

Instructions for Use

See cell line data sheet for detailed culturing and assay protocol.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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TIGIT - HEK293 Recombinant Cell Line

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