TCR Activator Raji Cell Line

Catalog #
60556
$11,095 *
Size: 2 vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Recombinant Raji cell line stably expressing an engineered, membrane-bound T cell receptor (TCR) activator. The cell line has been functionally validated in a co-culture assay for activation of the NFAT Reporter Jurkat cells.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Product Info
Storage and Usage
Citations
Host Cell Line
Raji, a human B lymphoblastoid cell line derived from a patient with Burkitt’s lymphoma, suspension
Supplied As
Each vial contains 1 x 106 cells in 1 ml of cell freezing medium (BPS Bioscience #79796)
Materials Required But Not Supplied
Name Ordering Information
Thaw Medium 2 BPS Bioscience #60184
Growth Medium 2D BPS Bioscience #79639
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The T Cell Receptor (TCR) is a heterodimer protein found at the surface of T cells, which is stimulated upon binding to Major Histocompatibility Complex (MHC)/peptide complexes and triggers a signaling cascade that leads to the activation of transcription factors involved in the upregulation and secretion of cytokines, T cell proliferation, and cell differentiation into effector and memory cells.

The Raji line was established from a Burkitt's lymphoma patient. Raji cells constitutively express B cell antigens CD19, CD20, and CD22, and offer a physiologically relevant platform to evaluate cancer-directed immunotherapies such as Chimeric Antigen Receptor (CAR) T-cells. Raji Recombinant Cell Lines are biologically relevant targets for CAR-T or NK cells targeting CD19, CD20, or CD22.

When co-cultured with T cells, the engineered TCR activator cells stimulate the TCR on T cells, leading to activation of downstream signaling pathways and biological responses such as proliferation.