Recombinant stable clonal CHO cell line constitutively expressing full length human LIGHT protein, also known as TNFSF14, (Genbank #NM_003807). Surface expression is confirmed by flow cytometry.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
UniProt #
O43557
Mycoplasma Testing
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Background
LIGHT (TNFSF14) is a key component of the communication system that controls the responses of T-Cells. LIGHT activates two cell surface receptors, the Herpesvirus Entry Mediator (HVEM) and the Lymphotoxin-beta Receptor (LTβR). The LIGHT-HVEM axis is an important costimulatory pathway for T cells, it has been shown to activate NF-κB; LIGHT/HVEM pair are co-stimulatory immune checkpoint molecules for cancer immunotherapy, LIGHT expressing cells have been shown to be more potent than the soluble LIGHT for activating HVEM expressed on T cell surface.
References
1. Chen, et.al. (2013) Molecular mechanisms of T cell co-stimulation and co-inhibition. Nat Rev Immunol. 2013 April ; 13(4): 227–242.
2. Steinberg, et.al. (2014) The Signaling Networks of the Herpesvirus Entry Mediator (TNFRSF14) in Immune Regulation. Immunol Rev. 2011 November; 244(1): 169–187.
3. Rio, et.al. (2014) Therapeutic blockade of LIGHT interaction with HVEM and LTβR attenuates in vivo cytotoxic allogeneic responses. Transplantation. 2014 December 15; 98(11): 1165–1174.
Storage/Stability
Store in liquid nitrogen immediately upon receipt.
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.