IDO2 Cellular Activity QuickDetect™ Supplements

Catalog #
62001-2
$1,095 *
Size: 1000 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The IDO2 Cellular Activity QuickDetectTM Supplements are designed to complement the hIDO2-HEK293 Recombinant Cell Line (BPS Bioscience #60533) or other IDO2-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO2 and for the analysis and detection of Indoleamine 2,3 dioxygenase 2 (IDO2)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.

Synonyms
Indoleamine 2,3-dioxygenase 2, IDO2
Product Info
Storage and Usage
Citations
Materials Required But Not Supplied
Name Ordering Information
hIDO2-HEK293 Recombinant Cell Line or other IDO2-expressing cell line and appropriate cell culture medium BPS Bioscience #60533
INCB024360 or other IDO2 inhibitor BPS Bioscience #27339
6.1 N (concentrated) trichloroacetic acid*  
17.4 N (concentrated) glacial acetic acid*  

*Note: both trichloroacetic acid and acetic acid are strongly corrosive acids; please use gloves and appropriate protective clothing.

Format
Component Amount Storage
IDO2 Assay Medium Supplement 1 2 x 1 ml 4°C
IDO2 Assay Medium Supplement 2 4 x 500 µl -20°C
Detection Reagent 2 g Room Temp.
Background

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells. The L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp. Additionally, the released degradation products of L-Trp have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors and provide cancer cells with an avenue for immune evasion.

References
1. Metz, R., et al., Int. Immunol. 2014; 26: 357–367.

2. Fatokun, A., et al., Amino Acids 2013; 45: 1319-1329.