IDO1 Cellular Activity QuickDetect™ Supplements

Catalog #
62000-2
$1,095 *
Size: 1000 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The IDO1 Cellular Activity QuickDetectTM Supplements are designed to complement the hIDO1-HEK293 Recombinant Cell Line (BPS Bioscience #60532) or other IDO1-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO1 and for the analysis and detection of Indoleamine 2,3 dioxygenase 1 (IDO1)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.

Synonyms
Indoleamine 2,3-dioxygenase 1, IDO
Product Info
Storage and Usage
Citations
Materials Required But Not Supplied
Name Ordering Information
hIDO1-HEK293 Recombinant Cell Line or other IDO1-expressing cell line and appropriate cell culture medium BPS Bioscience #60532
INCB024360 or other IDO1 inhibitor BPS Bioscience #27339
6.1 N (concentrated) trichloroacetic acid*  
17.4 N (concentrated) glacial acetic acid*  

*Note: both trichloroacetic acid and acetic acid are strongly corrosive acids; please use gloves and appropriate protective clothing.

Format
Component Amount Storage
IDO1 Assay Medium Supplement 1 2 x 1 ml 4°C
IDO1 Assay Medium Supplement 2 4 x 500 µl -20°C
Detection Reagent 2 g Room Temp.
Background

L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. IDO1 is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

References

1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.