IDO1 Cellular Activity QuickDetect™ Supplements
The IDO1 Cellular Activity QuickDetectTM Supplements are designed to complement the hIDO1-HEK293 Recombinant Cell Line (BPS Bioscience #60532) or other IDO1-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO1 and for the analysis and detection of Indoleamine 2,3 dioxygenase 1 (IDO1)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
Name | Ordering Information |
hIDO1-HEK293 Recombinant Cell Line or other IDO1-expressing cell line and appropriate cell culture medium | BPS Bioscience #60532 |
INCB024360 or other IDO1 inhibitor | BPS Bioscience #27339 |
6.1 N (concentrated) trichloroacetic acid* | |
17.4 N (concentrated) glacial acetic acid* |
*Note: both trichloroacetic acid and acetic acid are strongly corrosive acids; please use gloves and appropriate protective clothing.
Component | Amount | Storage |
IDO1 Assay Medium Supplement 1 | 2 x 1 ml | 4°C |
IDO1 Assay Medium Supplement 2 | 4 x 500 µl | -20°C |
Detection Reagent | 2 g | Room Temp. |
L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. IDO1 is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.