IDO1 Cellular Activity QuickDetect™ Supplements

Catalog #: 62000-1
Size: 100 reactions
$205
Background:
L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of LTrp that have immunosuppressive functions. IDO1 is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description:
The IDO1 Cellular Activity QuickDetectTM Supplements are designed to complement the hIDO1-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO1- expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO1 and for the analysis and detection of Idoleamine 2,3 dioxygenase 1 (IDO1)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
Materials Required But Not Provided:

hIDO1-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO1-expressing cell line and appropriate cell culture medium
INCB024360 (BPS Cat. #27339) or other IDO1 inhibitor
6.1 N (concentrated) trichloroacetic acid*
17.4 N (concentrated) glacial acetic acid*
*Note: both trichloroacetic acid and acetic acid are strongly corrosive acids; please use gloves and appropriate protective clothing.

Synonym(s):
Indoleamine 2,3-dioxygenase 1, IDO
Format:

Instructions for use:
see data sheet for detailed culturing and assay protocol.
Storage / Stability:
Stable for at least 6 months when stored as directed
Application(s):
Monitor IDO1 pathway activity. Screen for activators or inhibitors of IDO1 in a cellular context
Reference(s):

1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.

Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

62000-1.pdf