IDO1 Cell-Based Assay Kit

Catalog #
72031
$595 *
Size: 100 reactions
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Description

The human IDO1 Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human IDO1 in cultured cells.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
Indoleamine 2,3-dioxygenase 1, IDO, IDO2, IDO Assay IDO1 Assay
Product Info
Storage and Usage
Citations
Supplied As
The kit contains a transfection-ready expression vector for full length human IDO1 (hIDO1). Upon expression, hIDO1 catalyzes L-Trp conversion to Kyn, which gets released and is detected in the assay medium. The kit also includes specialized assay medium.
Format

COMPONENTS:

UniProt #
P14902
Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of LTrp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
References

1. Yue, E., et al., J. Med. Chem. 2009; 52: 7364–7367.
2. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.

Application References:
1. Deep Learning Model Enables the Discovery of a Novel Immunotherapeutic Agent Regulating the Kynurenine Pathway (2021)