HVEM/NF-κB Reporter Jurkat Recombinant Cell Line

Catalog #: 79310
Size: 2 vials
$8,000
Background:
HVEM is a bidirectional switch regulating T-cell activation in a costimulatory or coinhibitory fashion whose outcome depends on the binding partner. HVEM can act as both receptor and ligand, the binding of endogenous ligand LIGHT or agonist antibodies to HVEM delivers a costimulatory signal; whereas the binding of HVEM to BTLA (IgSF) or CD160 on Effector T cells delivers a coinhibitory signal. LIGHT/HVEM axis are co-stimulatory immune checkpoint molecules extensively studied for cancer immunotherapy; LIGHT, either in the soluble monomer form or homotrimer expressed on the cell surface, can activate NF-kB through binding to HVEM; we have shown that LIGHT expressing cells are more potent and efficacious than the soluble LIGHT for activating HVEM expressed on T cell surface.
Description:

Recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-κB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, CD270, GenBank Accession No. NM_003820). Following activation by human HVEM ligand LIGHT, NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene.

Materials Required But Not Provided:
Synonym(s):
HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR
Supplied As:
Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO.
Host Cell line:
Jurkat T cell
Mycoplasma Testing:
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 2 (BPS Cat. #60184): RPMI1640 medium (Life Technologies #A10491-01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01)

Complete Growth Medium: Thaw Medium 2 (BPS Cat. #60184) plus 1 mg/ml G418 (Thermo Fisher, Cat. # 11811031) and 200 μg/ml of Hygromycin B (Hyclone #SV30070.01)

Cells should be grown at 37°C with 5% CO2 using complete growth medium (Thaw Medium 2 with G418 and Hygromycin B).

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
•Screen for agonists or antagonists of LIGHT-HVEM signaling in a physiological relevant cellular context

•Characterize T cell-mediated immune responses of HVEM and its interactions with LIGHT

•Screen co-stimulatory immune checkpoint molecules

Notes:

Contact us for pricing.

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

79310.pdf