HVEM - HEK293 Recombinant Cell Line

Catalog #

79313

$4,400 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant HEK293 stably expressing human HVEM (Herpesvirus entry mediator; CD270; TNFRSF14; ATAR) GenBank Accession # NM_003820).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Immunotherapy, CD270, Herpesvirus entry mediator, HVEM, HEK293, HVEM - HEK293, TNFRSF14, BTLA

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Product Data Gallery

Flow cytometry analysis of cell surface expression of HVEM in HVEM-HEK293 cells.

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293 cells

Supplied As

Each vial contains ~3 X 10⁶ cells in 1 ml of 10% DMSO.

UniProt #

Q92956

Mycoplasma Testing

The cell line has been screened using the metabolite-based Mycoplasma Detection Kit (Biotool, #B3903) to confirm the absence of Mycoplasma species.

Background

HVEM (CD270, TNFRSF14) is a human cell surface receptor in the TNF-receptor superfamily that can act as both a co-stimulatory receptor and a co-inhibitory receptor of T cells. Binding of HVEM to one of its ligands, LIGHT (CD258, TNFSF14) or LTα (lymphotoxin-α), causes a co-stimulatory signal which can activate lymphoid cells. Alternately, interaction with BTLA (CD272) or CD160 causes a co-inhibitory signal which negatively regulates T-cell immune responses. HVEM has also been shown to interact with adaptor proteins TRAF2 and TRAF5, and is critical to herpes simplex virus (HSV) cellular entry.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS, #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1F (BPS, #79540): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 100 µg/ml Hygromycin B

Instructions for Use

See cell line data sheet for detailed culturing and assay protocol.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only