HEK293 - T-REx Recombinant Cell Line

Catalog #: 71227
Size: 2 vials
$1,025
Species: Human
Host Species: HEK293
Background:
The T-REx cell line expresses the tetracycline repressor protein. Cells are transfected with the gene of interest under control of a minimal promoter and the tet operon. The Tet repressor protein binds to the operon and represses transcription. Tetracycline regulation is based on the binding of either tetracycline (or doxycycline) to the Tet repressor protein, which de-represses the promoter controlling expression of the gene of interest.
Description:
Recombinant HEK293 cell transfected with tetracycline repressor gene. This cell line is validated for response to the stimulation of doxycycline.
Culture Conditions:
Cells should be grown at 37°C with 5% CO2 using MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acid (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01), and 5 ug/ml of Blasticidin (Invitrogen #R210-01). If culturing cells in medium from other vendors, it may be required to lower the percentage of CO2 in the incubator depending on the NaHCO3 level in the basal medium.

It is recommended to quickly thaw the frozen cells from liquid nitrogen in a 37°C water-bath, transfer to a tube containing 10 ml of growth medium without Blasticidin, spin down cells, resuspend cells in pre-warmed growth medium without Blasticidin, transfer resuspended cells to T25 flask and culture at 37°C in CO2 incubator. The next day, replace the medium with fresh growth medium without Blasticidin, and continue growing culture in a CO2 incubator at 37°C until the cells are ready to be split. Cells should be split before they reach complete confluence. At first passage switch to growth medium containing Blasticidin. Cells should be split before they reach complete confluence.

To passage the cells, rinse cells with phosphate buffered saline (PBS), detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels.

Format:
Each vial contains 1.5 X 10^6 cells in 1 ml of 10% DMSO
Reference(s):
On the use of the T-REx tetracycline-inducible gene expression system in vivo. Dobrovolsky VN1, Heflich RH. 2007. Biotechnol Bioeng. 98(3):719-23.
Notes:

Just after thawing and at low density, the cells may grow at a slower rate. It is recommended to split the cells with ~ 1:4 ratio at the beginning of culturing. After several passages, the cell growth rate increases and the cells can be split with 1:8 -1:20 ratio weekly.

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line.  Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license; contact sales@bpsbioscience.com for details.  Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

 

Scientific Category:
Immunotherapy
Product Type:
Cell Line
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71227.pdf