CTLA4 / IL-2 Reporter - Jurkat Recombinant Cell Line

Catalog #: 79525
Size: 2 vials
CTLA4, also known as CD152, is a protein receptor that functioning as an immune checkpoint. It is expressed by activated T cells and transmits an inhibitory signal to T cells. CTLA4 is homologous to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells. CTLA-4 binds to CD80 and CD86 with greater affinity and avidity than CD28 thus enabling it to outcompete CD28 for its ligands and act as an “off” switch when bound to CD80 or CD86. CTLA-4 is an important immunotherapy target for the treatment of cancer and autoimmune diseases.
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of IL-2 promoter with constitutive expression of human CTLA4 (Cytotoxic T-Lymphocyte Associated Protein, CD152; GenBank Accession #NM_005214).
Materials Required But Not Provided:

• Raji cell (ATCC # CCL-86)

Thaw Medium 2 (BPS Bioscience #60184)

Growth Medium 2A (BPS Bioscience #60190)

• Assay medium: RPMI1640 medium (Life Technologies #A10491-01)

Anti-CD3 agonist antibody (BPS bioscience #71274)

• Anti-CTLA4 neutralizing antibody (Biolegend #349904, clone#L3D10)

• 96-well tissue culture-treated white clear-bottom assay plate

One-Step luciferase assay system (BPS bioscience # 60690) or other luciferase reagents for measuring firefly luciferase activity

• Luminometer

Supplied As:
Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Host Cell line:
Jurkat T
Mycoplasma Testing:

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.

Growth Media:

Thaw Medium 2 (BPS Cat. #60184): RPMI1640 medium (Life Technologies #A10491-01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01)

Growth Medium 2A (BPS Cat. #60190):  Thaw Medium 2 (BPS Cat. #60184) plus 1 mg/ml of Geneticin (Life Technologies #11811031) and 200 μg/ml of Hygromycin B (Life Technologies #10687-010).

Instructions for use:
See data sheet for detailed culturing and assay protocol
Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
•Screen for activators or inhibitors of CTLA4 signaling in a cellular context
•Characterize the biological activity of CTLA4 and its interactions with ligands
License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
Avoid freeze/thaw cycles
Scientific Category:
Product Type:
Cell line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets