CTLA4 - HEK293 Recombinant Cell Line

Catalog #

60681

$4,400 *

Size: 2 vials

Qty

Bulk/Custom

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Description

Recombinant HEK293 stably expressing human CTLA4 (Cytotoxic T-Lymphocyte Associated Protein; CD152), GenBank Accession #NM_005214.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Cytotoxic T-lymphocyte-associated protein 4, CD152, CTLA-4

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Product Data Gallery

Flow cytometry analysis of cell surface expression of CTLA4 in CTLA4-HEK293 cells

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293 cells

Supplied As

Each vial contains ~3 X 10⁶ cells in 1 ml of 10% DMSO.

UniProt #

P16410

Mycoplasma Testing

The cell line has been screened using the metabolite-based Mycoplasma Detection Kit (Biotool, #B3903) to confirm the absence of Mycoplasma species.

Background

CTLA4 is a member of the immunoglobulin superfamily. It is expressed by activated T cells and transmits an inhibitory signal to T cells. CTLA4 is homologous to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells. CTLA-4 binds CD80 and CD86 with greater affinity and avidity than CD28 thus enabling it to outcompete CD28 for its ligands and act as an “off” switch when bound to CD80 or CD86. Not surprisingly, CTLA-4 is an important drug target for the regulation of the host’s response to cancer.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

Thaw Medium 1 (BPS Bioscience, #60187): MEM medium (Hyclone, #SH30024.01) + 10% FBS (Life Technologies, #26140-079) + 1% non-essential amino acids (Hyclone, #SH30238.01) + 1 mM Na pyruvate (Hyclone, #SH30239.01) + 1% Penicillin/Streptomycin (Hyclone, SV30010.01)

Growth Medium 1F (BPS Bioscience #79540): Thaw Medium 1 (BPS Bioscience, #60187) plus 100 µg/ml of Hygromycin B (Life Technologies, #10687-010).

Cells should be grown at 37°C with 5% CO₂ using Growth Medium 1F to ensure recombinant expression. CTLA4-HEK293 cells should display a typical cell division time of about 24 hours.

Instructions for Use

See cell line data sheet for detailed culturing and assay protocol.

Shipping Temperature

-80°C (dry ice)

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only