CD47 CHO-K1 Cell line

Catalog #: 60602
Size: 2 vials
$3,850
Background:

CD47 (also known as Rh-associated protein, GP42, integrin-associated protein (IAP), and neurophilin) is an immunoglobulin-like protein that interacts with its receptor, Signal-regulatory protein alpha (SIRPα), on monocytes, macrophages or dendritic cells. This binding interaction regulates transmigration, oxidative burst cytokine production, and phagocytosis, creating a “don’t eat me” signal. CD47 is ubiquitously expressed on the surface of normal cells, but is overexpressed in numerous cancer cells, where it is thought to contribute to the resistance of tumors to phagocyte-dependent clearance.

Description:
Recombinant CHO-K1 cell that constitutively expresses full length human CD47 (NM_198793.2). Surface expression is confirmed by flow cytometry.
Host Cell line:
CHO-K1
Mycoplasma Testing:
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 3 (BPS Cat. #60186): Ham’s F-12 medium (Hyclone # SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 3B (BPS Cat. #79529): Thaw Medium 3 (BPS Cat. #60186) plus 500 μg/ml of Hygromycin B (Hyclone #SV30070.01) to ensure the recombinant expression.

Format:
Each vial contains ~3 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
This cell line is ideal for CD47 binding assays.
Reference(s):
1. Weiskopf K et.al. (2016) CD47-blocking immunotherapies stimulate macrophage-mediated destruction of small-cell lung cancer. J Clinical Investigation. 126:2610-2620.

2. Zhao XW et.al. (2011) CD47-signal regulatory protein (SIRP-alpha) interactions form a barrier for antibody-mediated tumor cell destruction. PNAS. 108: 18342-18347.

3. Piccio L et.al. (2005) Adhesion of human T cells to antigen-presenting cells through SIRPβ2-CD47 interaction co-stimulates T-cell proliferation. Blood. 105: 2421-2427.

Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60602.pdf