CD27/NF-kB Reporter-Jurkat Recombinant Cell Line

Catalog #

79509

$10,175 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NF-kB response elements with constitutive expression of human CD27 (also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, Genbank Accession #BC012160)

Interested in screening and profiling inhibitors, blocking antibodies, or activators of CD27 without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, T14, cd27 cell line, cd-27, nfkb, nf-kb

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Product Data Gallery

The CD27/NF-B Reporter Activities Stimulated by CD70-CHO Cells

Anti-CD27 Blocking Antibody Dose Response in Co-Cultured CD27/NFB Reporter-Jurkat and CD70-CHO cells

Dose response of anti-CD27 agonist antibody on CD27/NF-B Reporter-Jurkat cells cocultured with FcGR2B CHO K1 cells

FACS Analysis of Cell Surface Expression of CD27 in CD27/NF-B Reporter-Jurkat cells

Product Info

Storage and Usage

Citations

Host Cell Line

NF-kB

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS.

Materials Required But Not Supplied

Assay Medium: Thaw Medium 2 (BPS Bioscience #60184)
Growth Medium 2C (BPS Bioscience #79592)
CD70-CHO K1 Recombinant Cell Line (BPS Bioscience #79510)

FcGR2B-CHO K1 Recombinant Cell Line (BPS Bioscience #79511)

96-well tissue culture-treated white clear-bottom assay plate
One-Step luciferase assay system (BPS Bioscience #60690) or other luciferase reagents for measuring firefly luciferase activity
Luminometer

UniProt #

P26842

Mycoplasma Testing

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination.

Background

CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the T cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). CD27 is expressed on various types of T cells, B cells and a subset of natural killer cells. It activates NF-κB and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed by numerous tumor cells. Adaptor proteins TRAF2 and TRAF5 can also stimulate CD27 signaling. Activation of CD27 leads to lymphoid proliferation, differentiation, apoptosis, and the induction of long-term memory. The CD27/CD70 pathway is a key target for the development of treatments for cancer and inflammatory diseases.

Storage/Stability

Store in liquid nitrogen immediately upon receipt

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 2C (BPS #79592): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin, 1 mg/ml of Geneticin, and 100 μg/ml of Hygromycin B

Instructions for Use

See data sheet for detailed culturing and assay protocol

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only