CD137 (4-1BB) HEK293 Recombinant Cell Line

Catalog #

60691

$4,400 *

Size: 2 vials

Qty

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Description

Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_001561). Surface expression is confirmed by flow cytometry.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

4-1BB, TNFRS9, Mitogen-Activated Protein Kinase 14, Signal Transducer And Activator Of Transcription 3, STAT3, MAPK14

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Product Data Gallery

Expression of CD137 (4-1BB) protein validated by flow cytometry

Product Info

Storage and Usage

Citations

Host Cell Line

Human Embryonic Kidney cell line (HEK293). Adherent epithelial cells.

Species

Human

Supplied As

Each vial contains ~ 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS.

Genbank #

NM_001561

UniProt #

Q07011

Mycoplasma Testing

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.

Background

Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients.

References

1. McNamara II, J.O., et.al. (2008) Multivalent 4-1BB binding aptamers co-stimulate CD8+ T cells and inhibit tumor growth in mice. J. Clin. Invest. 118: 376-386.

2. Ma, B.Y., et.al. (2005) The expression and regulatory role of OX40 and 4-1BB heterodimers in activated human T cells. Blood. 106: 2002-2010.

Storage/Stability

Immediately upon receipt, store vials in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS, #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1F (BPS, #79540): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 100 µg/ml Hygromycin B

Instructions for Use

See data sheet for detailed culturing and assay protocol

Shipping Temperature

-80°C (dry ice)

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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CD137 (4-1BB) HEK293 Recombinant Cell Line

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