ADCC Bioassay Effector Cell (Mouse) - Jurkat Recombinant Cell Line

Catalog #: 79733
Size: 2 vials
Species: Mouse
The mouse ADCC Reporter cells (mFcγRIV /NFAT-Jurkat cells) are recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of mouse FcγRIV (NM_144559.2).
Materials Required But Not Provided:

Assay Medium 2A (BPS Bioscience, #79621): RPMI 1640 medium (Thermo Fisher, #A1049101) supplemented with 10% low IgG FBS (Thermo Fisher, #16250078), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).
• Anti-CD20 mouse IgG2a (InvivoGen #hcd20-mab10)
• Human B cell WIL2-S (ATCC #CRL-8885)
• 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690)
• Luminometer

Antibody-dependent cell-mediated cytotoxicity, natural killer bioassay, NK cell bioassay
Host Cell line:
NFAT-Jurkat cells
Mycoplasma Testing:
This cell line has been screened using the Venor™ GeM Mycoplasma Detection Kit, PCR Based (Sigma, #MP0025) to confirm the absence of Mycoplasma contamination.
Growth Media:

Thaw Medium 2 (BPS Cat. #60184): RPMI1640 medium (Life Technologies, #A10491-01) supplemented with 10% FBS (Life Technologies, #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Two vials containing ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS.
Instructions for use:
See data sheet for detailed instruction and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt. Do not store for long-term at -80°C or on dry ice.
Characterize the Fc effector function of antibodies and measure ADCC activity in cellular assays.

Bhavin S. Parekh, et al. 2012. “Development and validation of an antibody-dependent cellmediated
cytotoxicity-reporter gene assay.” mAbs, 4(3):310-318 , doi:10.4161/mabs.19873

Carlos Rosales. 2017. “Fcγ Receptor Heterogeneity in Leukocyte Functional Responses.”
Frontiers in Immunology, 8: 280, doi:10.3389/fimmu.2017.00280

Avoid multiple freeze/thaw cycles.
Scientific Category:
Product Type:
Cell Line
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