ADCC Bioassay Effector Cell (Mouse) Jurkat Cell Line

Catalog #
79733
$6,250 *
Size: 2 vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The ADCC Bioassay Effector Cell (Mouse) Jurkat Cell Line (mFcγRIV /NFAT-Jurkat cells) are engineered Jurkat cells that express firefly luciferase, under the control of NFAT response elements, and mouse FcγRIV (NM_144559.2). This cell line was functionally validated in an ADCC (antibody-dependent cell-mediated cytotoxicity) assay. 

Figure 1: Illustration of the mechanism of action of ADCC Bioassay Effector Cell (Mouse) Jurkat Cell Line. ADCC Bioassay Effector Cell (Mouse) Jurkat cells are used as effector cells. The Fc effector portion of antibodies binds to target antigens on the target cell surface and also binds to mFcγRIV on the cell surface of effector cells, cross-linking of the effectors and target cells. Binding to mFcγRIV leads to the activation of NFAT pathway in the effector cells. Luciferase activity is proportional to the amount of crosslinking and activation of the ADCC cascade.

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This product has been cited 2 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
Antibody-dependent cell-mediated cytotoxicity, natural killer bioassay, NK cell bioassay, Fc gamma receptor, Fcgamma receptor
Product Info
Storage and Usage
Citations2
Host Cell Line
Jurkat is a human leukemia cell line, Non-adherent T lymphocytes.
Species
Mouse
Supplied As
Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Materials Required But Not Supplied

Media Required for Cell Culture

Name Ordering Information
Thaw Medium 2 BPS Bioscience #60184
Growth Medium 2G BPS Bioscience #79734


Materials Required for Cellular Assays

Name Ordering Information
Assay Medium 2A BPS Bioscience #79621
Anti-CD20 Mouse IgG2a InvivoGen #hcd20-mab10
Human B Cells WIL2-S ATCC #CRL-8885
96-well tissue culture-treated white clear-bottom assay plate  
One-Step™ Luciferase Assay System BPS Bioscience #60690
Luminometer  
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune defense mechanism involving an effector immune cell lysing a target cell on which antibodies have bound to specific antigens. The typical ADCC involves activation of natural killer (NK) cells by antibodies engaged on a target. NK cells express Fc receptors, mostly FcγRIV in mouse, on their cell surface. These Fc receptors recognize and bind to the Fc portion of antibodies, usually IgG2a, IgG2b and IgG3 in mouse, present on the surface of a pathogen-infected target cell. Once the Fc receptor binds to the Fc region of IgG, the NK cell releases cytokines such as IFN-γ and cytotoxic molecules that attack the pathogen-infected target cell.

References

Parekh B., et al. 2012. mAbs 4(3):310-318, doi:10.4161/mabs.19873
Rosales C., 2017. Frontiers in Immunology 8: 280, doi:10.3389/fimmu.2017.00280