CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit

Catalog #
79257
$745 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD40:CD40L signaling. This kit comes in a convenient 96-well format, with biotin-labeled CD40, purified CD40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD40L is coated on a 96-well plate. Next, CD40 is incubated with CD40L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Synonyms
T cell antigen Gp39, TNF related activation protein, TRAP, tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, CD40, CD40 Molecule, activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
This kit comes in a convenient 96-well format, with biotin-labeled CD40, purified CD40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied
  • PBS (Phosphate buffered saline)
  • Luminometer or microplate reader capable of reading chemiluminescence
  • Rotating or rocker platform
Format
Catalog # Component Amount Storage
79102 CD40, Biotin-labeled 50 µg -80 °C Avoid
multiple

freeze/ thaw 
cycles
71191 CD40L-His 10 µg -80 °C
79311 3x Immuno Buffer 1 50 ml -20 °C
79728 Blocking Buffer 2 50 ml +4 °C
79742 Streptavidin-HRP 10 µl -20 °C
79670 ELISA ECL substrate A(transparent bottle) 6 ml Room Temp.
ELISA ECL substrate B
(brown bottle)
6 ml Room Temp.
79699 96-well white microplate 1 +4 °C

 

 

 

 

 

 

 

 

 

 

 

 

 

 

*The concentrations of CD40 and CD40L are lot-specific and will be indicated on the tubes containing the protein.

UniProt #
P29965