BAFF:BCMA[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

79667

$770 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The BAFF:BCMA[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BAFF:BCMA signaling.The key to this kit is the high sensitivity of detection of biotin-labeled BCMA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BAFF is coated on a 96-well plate. Next, BCMA is incubated with BAFF on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against BAFF:BCMA[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

BAFF, BLYS, TALL-1, CD257, TNFRSF13C, B-cell activating factor, TNF13b

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96- well format, with biotin-labeled BCMA, purified BAFF, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format

Catalog #	Component	Amount	Storage
100194	BAFF, His-Avi-tag HiP™	10 µg	-80°C	Avoid freeze/ thaw cycles
79467	BCMA, Fc-fusion (IgG1), Avi-Tag, Biotin-Labeled HiP™	5 µg	-80°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer	50 ml	+4°C
79742	Streptavidin-HRP	10 µl	-20°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp
79699	96-well white microplate	1	+4°C

UniProt #

BAFF: Q9Y275; BCMA: Q02223

Background

The TNF family ligand B-cell Activating Factor (BAFF, also known as BLyS, TALL-1 or CD257, encoded by the TNFSF13B gene) is a Type II membrane-bound protein, which can be released as a soluble ligand upon proteolytic processing. This cytokine is a ligand for the receptors Transmembrane Activator and CAML Interactor (TACI, TNFRSF13B), BAFF Receptor (BAFF-R, TNFRSF13C) and B-cell Maturation Antigen (BCMA, TNFRSF17). These interactions promote cell survival and they play a crucial role in B cell development. In particular, BCMA signaling is an important player in the later stages of B cell differentiation, including the survival of long-lived bone marrow plasma cells and likely for the survival of plasmablasts.

References

Thompson, J. S., et al. J. Exp. Med. 2000, 192(1): 129-136

Bossen, C., et al. Semin. Immunol. 2006, 18(5): 263-275

Storage/Stability

Up to 6 months from date of receipt, when stored as recommended.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only