Universal IDO1/IDO2/TDO Inhibitor Screening Assay Kit

Catalog #

72035

$925 *

Size: 150 reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The Universal IDO1/IDO2/TDO Inhibitor Screening Assay Kit is designed to measure IDO1, IDO2 and TDO enzyme inhibition.

This product has been cited 2 times.

●

Synonyms

activity assay kit

●

Product Data Gallery

Product Info

Storage and Usage

Citations2

Assay Kit Format

Colorimetric - UV Spectrophotometry

Supplied As

The kit comes in a convenient format with enough reaction solution and enzyme to perform a total of 50 reactions with each enzyme (150 reactions total). Inhibitor and enzyme are added to a sample containing L-Trp substrate.

Format

Catalog #	Component	Amount	Storage
71182	IDO1 His-Tag	20 µg	-80°C	Avoid Freeze/Thaw Cycles
73001	IDO1 Reaction Solution	10 ml	-80°C
73002	1x IDO1 Assay Buffer	1 ml	-80°C
71195	TDO, His-tag	25 µg	-80°C
73005	TDO Reaction Solution	10 ml	-80°C
73006	1x TDO Assay Buffer	1 ml	-80°C
71194	IDO2 His-Tag	500 µg	-80°C
	IDO2 Reaction Solution component 1	10 ml	-80°C
	IDO2 Reaction Solution component 2	100 µl	-80°C
	IDO2 Substrate	1 ml	-80°C
	1x IDO2 Assay Buffer	5 ml	-80°C
79965	UV transparent 96-well plate	2	Room Temp

UniProt #

P14902

Background

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2) and Tryptophan 2,3 dioxygenase (TDO) are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

References

1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.

2. Seegers, N., et al. J Biomol Screen. 2014; 19(9): 1266-74.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for the study of IDO1, IDO2, and TDO enzymology, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C (dry ice)

Contraindications

DMSO >0.5%, strong acids or bases, ionic detergents, and high salt.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only