TPH1 Inhibitor Screening Assay Kit

Catalog #

72053

$790 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple -just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

Need us to run inhibitor screens or profile your compounds against TPH1? Check out our Immunotherapy Biochemical Screening Services.

This product has been cited 3 times.

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Synonyms

tryptophan 5-hyrdoxylase, TRPH, activity assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations3

Assay Kit Format

Fluorogenic

Materials Required But Not Supplied

Microplate reader capable of measuring Fluorescence
Adjustable micropipettor and sterile tips

Format

Catalog Number	Component	Amount	Storage
71192	TPH1, His-tag	50 µg	-80°C	Avoid freeze/ thaw cycles!
	TPH Enzyme Solution	5 ml	-80°C
	TPH Reaction Solution	6 ml	-80°C
	TPH Quench Solution	1 ml	-20°C
79685	Black, 96 Well Plate	1	Room Temp.

UniProt #

P17752

Background

Tryptophan 5-hydroxylase 1 and 2 (TPH1 and TPH2) are enzymes that catalyze the monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP) which is subsequently converted to serotonin. By catalyzing the rate-limiting step in the biosynthesis of serotonin, these enzymes also play key roles in the signaling of other neurotransmitters downstream of serotonin synthesis, like melatonin. Additionally, TPH enzymes may play a role in immune regulation through tryptophan depletion.

References

1. Moran G. R., et al. J. Biol. Chem. 1998; 273:12259-12266.
2. Nowak E. C., et al., J. Exp. Med. 2012; 209:2127-2135.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See product datasheet for detailed protocol.

Applications

Useful for screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Metabolic Enzyme, Immunotherapy

Regulatory Status

Research Use Only