PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit

Catalog #

72006

$1,235 *

Size: 96 reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-L2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against PD-1[Biotinylated]:PD-L2? Check out our Immunotherapy Biochemical Screening Services.

●

Synonyms

activity assay kit, Strep HRP, streptavidin

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L2, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Format

Catalog Number	Component	Amount	Storage
71109	PD-1, Biotin-labeled	2 µg	-80°C	Avoid freeze/ thaw cycles!
71107	PD-L2	10 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp
79699	White 96-well microplate	1	+4°C

UniProt #

PD-1: Q15116; PD-L2: Q9BQ51

References

1. Lazar-Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Applications

This kit is most useful for screening for inhibitors of PD-L2.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cell Surface Receptor/Single-pass Transmembrane Protein

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit

PRODUCTS

SERVICES

RESOURCES

COMPANY