PD-1:PD-L2 TR-FRET Assay Kit
The PD-1:PD-L2 TR-FRET Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2 in a homogeneous 384 reaction format. Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L2, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
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This product has been cited 1 time.
Adjustable micropipettor and sterile tips.
Catalog Number | Component |
Amount |
Storage |
|
PD-1-Eu (Human) | 2 µg | -80°C |
|
|
71108 | PD-L2, Biotinylated (Human) | 20 µg | -80°C | |
Dye-labeled acceptor | 2 x 10 µl | -20°C | ||
79311 | 3x Immuno Buffer 1 | 4 ml | -20°C | |
White, non-binding, low volume, 384- well microtiter plate | 1 | Room temp. |
1. Molnar, E., et al. Proc Natl Acad Sci USA. 2008; 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008; 26: 677-704.