PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit

Catalog #
72003
$1,235 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-L1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L1 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

This product has been cited 2 times.

Synonyms
activity assay kit, Strep HRP, streptavidin
Product Info
Storage and Usage
Citations2
Assay Kit Format
Chemiluminescent
Supplied As
Kit comes in a convenient 96-well format, withbiotin-labeled PD-L1, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100binding reactions.
Materials Required But Not Supplied
Catalog # Component Amount Storage
71105 PD-L1, Biotin-labeled (Human) 5 µg -80°C Avoid
freeze/
thaw
cycles!
71106 PD-1 (Human) 10 µg -80°C
79742 Streptavidin-HRP 10 µl +4°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C
79670 ELISA ECL substrate A
(transparent bottle)
6 ml Room
Temp.
ELISA ECL substrate B
(brown bottle)
6 ml Room
Temp.
79699 White 96-well microplate 1 +4°C
UniProt #
PD-1: Q15116; PD-L1: Q9NZQ7
Background
Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses.
References

1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.