PD-1[Biotinylated]:PD-L1 Inhibitor Screening Colorimetric Assay Kit

Catalog #

72018

$1,095 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD- 1[Biotinylated]:PD-L1 Inhibitor Colorimetric Screening Assay Kit is designed for screening inhibitors of PD-1 receptor binding to PD-L1 ligand. TThe key to this kit is the high affinity of biotin-labeled PD-1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

Need us to run inhibitor screens or profile your compounds against PD-1[Biotinylated]:PD-L1? Check out our Immunotherapy Biochemical Screening Services.

This product has been cited 1 time.

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Synonyms

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1), activity assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations1

Assay Kit Format

Colorimetric

Supplied As

his kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Format

Catalog Number	Component	Amount	Storage
71109	PD-1, Biotin-labeled (Human)*	2 µg	-80°C	Avoid freeze/ thaw cycles
71104	PD-L1 (Human)*	10 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79651	Colorimetric HRP substrate	10 ml	+4°C
79964	Transparent 96-well microplate	1	+4°C

*The concentration of PD-1 and PD-L1 are lot-specific and will be indicated on the tubes containing the protein.

UniProt #

PD-1: Q15116; PD-L1: Q9NZQ7

References

1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

This kit is most useful for screening for inhibitors of PD-L1.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy & Cell Surface Receptors

Regulatory Status

Research Use Only

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PD-1[Biotinylated]:PD-L1 Inhibitor Screening Colorimetric Assay Kit

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