PD-1[Biotinylated]: PD-L1 Inhibitor Screening Assay Kit
Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1[Biotinylated]: PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. This kit comes in a convenient 384-well format with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for 400 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 384-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
PBS (Phosphate buffered saline) Luminometer or fluorescent microplate reader capable of reading chemiluminescence Rotating or rocker platform
Adjustable micropipettor and sterile tips
| Catalog # | Component | Amount | Storage | |
| 71109 | PD-1, Biotin-labeled | 2x2 µg | -80°C | Avoid freeze/ thaw cycles! |
| 71104 | PD-L1 | 2x10 µg | -80°C | |
| 79742 | Streptavidin-HRP | 2x10 µg | +4°C | |
| 79311 | 3x Immuno Buffer 1 | 2x50 µg | -20°C | |
| 79728 | Blocking Buffer 2 | 2x50 µg | +4°C | |
| 79670 | ELISA ECL substrate A (transparent bottle) |
2x6 ml | Room Temp |
|
| ELISA ECL substrate B (brown bottle) |
2x6 ml | Room Temp |
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| 78188 | White 384-well microplate | 1 | +4°C | |
1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.
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