Mouse PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit

Catalog #

72027

$1,300 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Mouse PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, mouse PD-L1 is coated on a 96-well plate. Next, mouse PD-1 is incubated with mouse PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against Mouse PD-1[Biotinylated]:PD-L1? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1), activity assay kit, PDL1

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Species

Mouse

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled mouse PD-1, purified mouse PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Format

Catalog #	Component	Amount	Storage
71117	Mouse PD-L1, Fc fusion*	30 µg	-80°C	Avoid freeze/ thaw cycles
71118	Mouse PD-1, Fc fusion, Biotin-labeled*	10 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
	3x Mouse PD-1 Assay Buffer	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp.
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp.
79699	White 96-well microplate	1	+4°C

*The concentrations of Mouse PD-L1 and Mouse PD-1 are lot-specific and will be indicated on the tubes containing the protein.

Background

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses for immune evasion.

References

1. Lin, D., et al. Proc. Natl. Acad. Sci. USA. 2008, 105: 3011-3016.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

This kit is perfect for screening for inhibitors of mouse PD-L1 binding to mouse PD-1.

Shipping Temperature

Dry ice

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy & Cell Surface Receptors

Regulatory Status

Research Use Only