MAT2A Inhibitor Screening Assay Kit

Catalog #
71402
$1,275 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The MAT2A Inhibitor Screening Assay Kit is designed to measure MAT2A activity for screening and profiling applications. The MAT2A assay kit comes in a convenient 384-well format, with purified recombinant MAT2A enzyme, L-Methionine, ATP, MAT2A assay buffer, and Colorimetric detection reagent for 384 enzyme reactions.

Synonyms
activity assay kit, colorimetric assay
Product Info
Storage and Usage
Citations
Assay Kit Format
Colorimetric
Materials Required But Not Supplied

UV/Vis spectrophotometer microplate reader capable of reading absorbance at 630 nm
Adjustable micropipettor and sterile tips
Rotating or rocker platform (optional)
Aluminum foil

Format
*Colorimetric Detection Reagent is used to measure the free phosphate from the MAT2A reaction. Any source of inorganic phosphate can interfere with the assay.
Catalog Number Components Amount Storage  
71401 MAT2A 250 µg -80°C



Avoid

freeze/
thaw
cycles!
  5x MAT2A Assay Buffer 3 ml -20°C
  ATP (750 μM) 1 ml -20°C
  L-Methionine (750 μM) 1 ml -20°C
74001 Colorimetric Detection Reagent* 2 x 10 ml +4°C
79962 Transparent 384-well plate 1 Room Temp.
UniProt #
P31153
Background
Methionine Adenosyltransferase 2A (MAT2A), also known as AdoMet Synthase 2, catalyzes the formation of S-adenosylmethionine from methionine and ATP. It is an important enzyme in cellular metabolism and is expressed in extrahepatic tissues. In liver, MAT2a expression is associated with growth, dedifferentiation, and cancer.
References

Frau, M., et al., Hepatology, 56 (1), 165-175 (2012)