IDO1 Inhibitor Screening Assay Kit

Catalog #

72021

$780 *

Size: 96 Reactions

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The IDO1 Inhibitor Screening Assay Kit is designed to measure IDO1 enzyme inhibition. The IDO1 Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=321 nm.

This product has been cited 3 times.

●

Synonyms

Indoleamine 2,3-dioxygenase 1, activity assay kit

●

Product Data Gallery

Product Info

Storage and Usage

Citations3

Assay Kit Format

Colorimetric - UV Spectrophotometry

Supplied As

The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.

Format

Catalog Number	Component	Amount	Storage
71182	IDO1 His-Tag	40 µg	-80°C	Avoid freeze/ thaw cycles!
73001	IDO1 Reaction Solution	2 x 10 ml	-80°C
73002	1x IDO1 Assay Buffer	1 ml	-80°C
79965	UV transparent 96-well plate	1	Room Temp

UniProt #

P14902

Background

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

References

1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
2. Seegers, N., et al. J Biomol Screen. 2014; 19(9): 1266-74.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

Useful for the study of IDO1 enzymology, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C (dry ice)

Contraindications

DMSO >0.5%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only