GITR:GITRL TR-FRET Assay Kit

Catalog #
79054
$1,095 *
Size: 384 reactions
Qty
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Description

The GITR:GITRL TR-FRET Assay is designed to measure the inhibition of GITR binding to GITRL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated GITR, GITRL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Synonyms
Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18
Product Info
Storage and Usage
Citations
Assay Kit Format
TR-FRET
Format
Catalog
Number

Component

Amount

Storage
71256 GITR, biotinylated 15 µg -80°C

Avoid
freeze/
thaw
cycles!
71190 GITRL-His 2 µg -80°C
Anti-His Tb Donor 2 x 10 µl -20°C
Dye-labeled Acceptor 2 x 10 µl -20°C
3x GITR TR FRET Assay Buffer 4 ml -20°C
White, non-binding, low volume, 384-
well microtiter plate

1
Room
Temp.
UniProt #
Q7TS55