GITR:GITRL TR-FRET Assay Kit
Catalog #
79054
$1,095
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Description
The GITR:GITRL TR-FRET Assay is designed to measure the inhibition of GITR binding to GITRL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated GITR, GITRL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
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Synonyms
Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18
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Product Data Gallery
Product Info
Storage and Usage
Citations
Assay Kit Format
TR-FRET
Format
Catalog Number |
Component |
Amount |
Storage |
|
71256 | GITR, biotinylated | 15 µg | -80°C | Avoid freeze/ thaw cycles! |
71190 | GITRL-His | 2 µg | -80°C | |
Anti-His Tb Donor | 2 x 10 µl | -20°C | ||
Dye-labeled Acceptor | 2 x 10 µl | -20°C | ||
3x GITR TR FRET Assay Buffer | 4 ml | -20°C | ||
White, non-binding, low volume, 384- well microtiter plate |
1 |
Room Temp. |
UniProt #
Q7TS55