GITR:GITRL TR-FRET Assay Kit

Catalog #: 79054
Size: 384 reactions
$1,065
Description:
The GITR:GITRL TR-FRET Assay is designed to measure the inhibition of GITR binding to GITRL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated GITR, GITRL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
UniProt
Q7TS55
Synonym(s):
Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, , HTRF
Format:
Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Notes:

1. Lechner, M.G., et al. Immunotherapy. 2011 Nov; 3(11): 1317–1340.

2. Ko, K., et al., J. Exp. Med. 2005; 202(7): 885-891.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Immunotherapy
Product Type:
Assay Kit
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Product Datasheets

79054.pdf