CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit

Catalog #: 72024
Size: 96 reactions
$1,045
Background:
B7-2 (CD86) signaling through CTLA4(CD152) has been shown to inhibit T cell activation. This co-inhibitory pathway can be overactive in many tumors, enabling cancers to escape the host’s immune system. CTLA4-blocking antibodies, including Ipilimumab (Yervoy) and Tremelimumab, have shown clinical efficacy in treating cancer.
Description:

The CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-2 interaction. The key to this kit is the high sensitivity of detection of biotin-labeled CTLA4 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, B7-2 is coated on a 96-well plate. Next, CTLA4-biotin is incubated with B7-2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Synonym(s):
T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, CD86, Cytotoxic T-lymphocyte-associated protein 4, CD152, B72, activity assay kit, Strep HRP, streptavidin
Supplied As:
This kit comes in a convenient 96- well format, with biotin-labeled CTLA4 (CD152), purified B7-2 (CD86), streptavidin-labeled HRP, and assay buffer for 100 binding reactions
Format:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Application(s):
This kit is useful for screening for inhibitors of CTLA4 binding to B7-2.
Reference(s):
1. Ohtani, H., et al., Lab Invest. 1997; 77(3): 231-241.
2. Rovert, C., et al., N. Engl. J. Med. 2011; 364: 2517-25262.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy/Cell Surface Receptor
Product Type:
Assay Kit
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Product Datasheets

72024.pdf