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Description
The CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-2 interaction. The key to this kit is the high sensitivity of detection of biotin-labeled CTLA4 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, B7-2 is coated on a 96-well plate. Next, CTLA4-biotin is incubated with B7-2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
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Synonyms
T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, CD86, Cytotoxic T-lymphocyte-associated protein 4, CD152, B72, activity assay kit, CTLA-4
Product Info
Storage and Usage
Citations
Supplied As
This kit comes in a convenient 96- well format, with biotin-labeled CTLA4 (CD152), purified B7-2 (CD86), streptavidin-labeled HRP, and assay buffer for 100 binding reactions
UniProt #
CTLA4: P16410; B7-2: P16410
Background
B7-2 (CD86) signaling through CTLA4(CD152) has been shown to inhibit T
cell activation. This co-inhibitory pathway can be overactive in many tumors, enabling cancers
to escape the host’s immune system. CTLA4-blocking antibodies, including Ipilimumab (Yervoy)
and Tremelimumab, have shown clinical efficacy in treating cancer.
References
1. Ohtani, H., et al., Lab Invest. 1997; 77(3): 231-241.
2. Rovert, C., et al., N. Engl. J. Med. 2011; 364: 2517-25262.
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Instructions for Use
See assay kit data sheet for detailed protocol
Applications
This kit is useful for screening for inhibitors of CTLA4 binding to B7-2.
Shipping Temperature
-80°C (dry ice)
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to
[email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
Scientific Category
Immunotherapy/Cell Surface Receptor
Regulatory Status
Research Use Only