CTLA4:B7-1 TR-FRET Assay Kit

Catalog #: 72120
Size: 384 reactions
$1,040
Description:
The CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of CTLA4 binding to B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated CTLA4, B7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Synonym(s):
T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, CD86, Cytotoxic T-lymphocyte-associated protein 4, CD152
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Huxley, P., et al. Chem Biol. 2004 Dec;11(12):1651-8.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy/Cell Surface Receptor
Product Type:
Assay Kit
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Product Datasheets

72120.pdf