CD73 Virus-Like-Particle (VLP) Inhibitor Screening Assay Kit

Catalog #: 79585
Size: 96 reactions
$995
Background:
CD73, also known as Ecto-5’-nucleotidase (5'-NT), promotes tumor growth and progression by degrading AMP into adenosine. CD73-generated adenosine blocks T-cell immuno-surveillance, resulting in an immunosuppressed and pro-angiogenic niche within the tumor microenvironment. CD73 functions in native systems as a dimer bound by a glycosyl phosphatidyl inositol lipid anchor that keeps the enzyme tethered to the extracellular face of the cell. This complex cannot easily be purified in its native form. Expression in VLPs allows for inhibitor screening of full length CD73.
Description:
The CD73 VLP Inhibitor Screening Assay Kit is designed to measure CD73 activity for screening and profiling applications. The CD73 assay kit comes in a convenient 96-well format, with full length CD73 enzyme in isolated VLPs, AMP, CD73 assay buffer, and Colorimetric detection reagent for 100 enzyme reactions.
Materials Required But Not Provided:
  • • UV/Vis spectrophotometer microplate reader capable of reading absorbance at 630 nm
  • • Adjustable micropipettor and sterile tips
  • • 37˚C incubator 
  • • Rotating or rocker platform (optional)
  • • Aluminum foil 
Synonym(s):
CD73, CD73 VLP, Virus Like Particle, Ecto-5’-nucleotidase, 5'-NT
Supplied As:
The CD73 assay kit comes in a convenient 96-well format, with full length CD73 enzyme in isolated VLPs, AMP, CD73 assay buffer, and Colorimetric detection reagent for 100 enzyme reactions.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

 Antonioli, L., et al., Trends in Cancer, Vol. 2, No. 2, 95-109

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

79585.pdf