CD73 Inhibitor Screening Assay Kit

Catalog #

72058

$1,245 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD73 Inhibitor Screening Assay Kit is designed to measure CD73 activity for screening and profiling applications. The CD73 assay kit comes in a convenient 384-well format, with purified recombinant CD73 enzyme, AMP, CD73 assay buffer, and Colorimetric detection reagent for 384 enzyme reactions.

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Synonyms

5'- nucleotidase, Ecto-5'-nucleotidase, activity assay kit, colorimetric assay

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Colorimetric

Materials Required But Not Supplied

UV/Vis spectrophotometer microplate reader capable of reading absorbance at 630 nm
Adjustable micropipettor and sterile tips
37˚C incubator Rotating or rocker platform (optional)
Aluminum foil

Format

Catalog Number	Component	Amount	Storage
71184	CD73	5 µg	-80°C	Avoid freeze /thaw cycles!
74000	5x CD73 assay buffer	3 x 1 ml	-20°C
79496	AMP (500 μM)	2 x 1 ml	-20°C
74001	Colorimetric detection reagent*	2 x 10 ml	+4°C
79962	Transparent 384-well plate	1	Room Temp.

*Colorimetric detection reagent is used to measure the free phosphate from the CD73 reaction. Any source of inorganic phosphate can interfere with the assay.

UniProt #

P21589

Background

CD39 contributes to immune regulation through the hydrolysis of ATP to AMP in the rate limiting step of extracellular adenosine production. CD39 is expressed on the surface of multiple cells of the immune system including T-cells, Natural killer cells, B-cells, dendritic cells and macrophages. In addition, high levels of CD39 expression have been associated with the tumor microenvironment (TME). Research aimed at reducing immunosuppression by CD39 activity has resulted in the study of a small molecule inhibitor, POM1, and multiple anti-CD39 antibodies selected for blocking enzymatic activity or decreasing CD39 expression.

References

Antonioli, L., et al., Trends in Cancer, Vol. 2, No. 2, 95-109.

Application Reference(s):
1. Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes (2017)

Storage/Stability

Up to 6 months from date of receipt, when stored as recommended.

Instructions for Use

See product datasheet for detailed assay protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Metabolic Enzyme, Immunotherapy

Regulatory Status

Research Use Only