CD73 Inhibitor Screening Assay Kit
The CD73 Inhibitor Screening Assay Kit is a 96-well colorimetric assay designed to measure the activity of CD73 for screening and profiling applications.
This product has been cited 2 times.
- UV/Vis spectrophotometer microplate reader capable of reading λ=630 nm
- Adjustable micropipettor and sterile tips
Catalog # | Name | Amount | Storage | |
71184 | CD73, His-Tag* | 5 µg | -80°C | Avoid freeze/ thaw cycles! |
74000 | 5x CD73 Assay Buffer | 2 x 1 ml | -20°C | |
79496 | 500 µM AMP | 1 ml | -20°C | |
74001 | Colorimetric Detection Reagent** | 10 ml | 4°C | |
79963 | Transparent 96-well plate | 1 | Room Temp |
*The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
**Colorimetric Detection Reagent is used to measure the free phosphate generated in the reaction catalyzed by CD73. Any other source of inorganic phosphate can interfere with the assay.
Cluster of Differentiation 73 (CD73), also known as ecto-5’-nucleotidase (NT5E) degrades AMP into adenosine. Adenosine is an anti-inflammatory molecule, and its regulation is crucial for immune system homeostasis. CD73 is expressed in several cell types, such as Treg cells and myeloid-derived suppressor cells. Upregulation of CD73 is linked to numerous cancer types, such as leukemia, glioblastoma, melanoma and ovarian and breast cancer. It is also linked to systemic lupus erythematosus. Its role in cancer makes CD73 inhibitors and antibodies promising therapeutical avenues for cancer and inflammatory diseases.
Antonioli, L., et al., Trends in Cancer, Vol. 2, No. 2, 95-109.