CD73 Inhibitor Screening Assay Kit

Catalog #: 72055
Size: 96 reactions
$765
Background:

CD39 contributes to immune regulation through the hydrolysis of ATP to AMP in the rate limiting step of extracellular adenosine production. CD39 is expressed on the surface of multiple cells of the immune system including T-cells, Natural killer cells, B-cells, dendritic cells and macrophages. In addition, high levels of CD39 expression have been associated with the tumor microenvironment (TME). Research aimed at reducing immunosuppression by CD39 activity has resulted in the study of a small molecule inhibitor, POM1, and multiple anti-CD39 antibodies selected for blocking enzymatic activity or decreasing CD39 expression. 

CD73/CD39 Immunotherapy

Description:
The CD73 Inhibitor Screening Assay Kit is designed to measure CD73 activity for screening and profiling applications. The CD73 assay kit comes in a convenient 96-well format, with purified recombinant CD73 enzyme, AMP, CD73 assay buffer, and Colorimetric detection reagent for 100 enzyme reactions.
Synonym(s):
5'- nucleotidase, Ecto-5'-nucleotidase, activity assay kit, colorimetric assay
Format:

COMPONENTS:

Instructions for use:
See product datasheet for detailed assay protocol.
Storage / Stability:
Up to 6 months from date of receipt, when stored as recommended.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 630 nm
Adjustable micropipettor and sterile tips
37°C incubator
Rotating or rocker platform
Aluminum foil
Warning(s):
*Colorimetric detection reagent is used to measure the free phosphate from the CD73 reaction. Any source of inorganic phosphate can interfere with the assay.
Avoid freeze/thaw cycles. 
Scientific Category:
Metabolic Enzyme, Immunotherapy
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

72055.pdf