CD47:SIRP-α[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

72044

$1,070 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD47:SIRP-α[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-α interaction. The key to this kit is the high sensitivity of detection of biotinlabeled SIRP-α by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate. Next, SIRP-α-biotin is incubated with CD47 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against CD47:SIRP-α[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

Cluster of Differentiation 47, in Tyrosine-protein phosphatase non-receptor type substrate 1, CD172a, Bit, and MyD-1, integrin associated protein (IAP), activity assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96- well format, with biotin-labeled SIRP-α, purified CD47, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Format

Catalog #	Componet	Amount	Storage
71177	CD47, Fc fusion	10 µg	-80°C	Avoid freeze/ thaw cycles
71138	SIRP-α (CD172a), His-tag, Biotin-labeled	60 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	+4°C
79670	ELISA ECL substrate B (brown bottle)	6 ml	+4°C
79699	White 96-well microplate	1	+4°C

UniProt #

CD47: Q08772; SIRP-α: P78324

Background

Signal-regulatory protein alpha (SIRP-α), also known as CD172a, is a cell surface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, is ubiquitously expressed on the surface of all cells and has been found to be overexpressed in some cancers. CD47 binding to SIRP-α delivers a “Do Not Eat Me” signal to macrophages. In the context of a tumor, this signaling aids in tumor evasion of the immune system. Inhibitors of this interaction hold great promise for cancer immunotherapy.

References

1. Chao, M.P., et al., Curr. Opin. Immunol. 2012; 24(2): 225-232.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

This kit is useful for screening for inhibitors of CD47 binding to SIRP-α.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only