CD40:CD40L TR-FRET Assay

Catalog #: 79258
Size: 384 reactions
$1,040
Description:
The CD40:CD40L TR-FRET Assay is designed to measure the inhibition of CD40 binding to CD40L in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated CD40, CD40L, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Synonym(s):
T cell antigen Gp39, TNF related activation protein, TRAP, tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, CD40, CD40 Molecule, HTRF
Supplied As:
This kit comes in a convenient 384-well format.
Format:
Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

79258.pdf