CD39 Virus-Like-Particle (VLP) Inhibitor Screening Assay Kit

Catalog #: 79583
Size: 96 reactions
$995
Background:
B cell activation marker CD39, also known as ecto-apyrase, ATP diphosphohydrolase, ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1) hydrolyzes extracellular nucleotide tri- and diphosphates in the presence of Ca++ and Mg++. It is an important enzyme in many biological processes, including the modulation of neural cell activities, prevention of intravascular thrombosis, and regulation of immune responses. CD39 functions in native systems as single pass transmembrane protein that keeps the enzyme domain on the extracellular face of the cell. This enzyme cannot easily be purified in its full length native form. Expression in VLPs allows for inhibitor screening of full length CD39.
Description:
The CD39 VLP Inhibitor Screening Assay Kit is designed to measure CD39 activity for screening and profiling applications. The CD39 VLP assay kit comes in a convenient 96-well format, with full length CD39 enzyme in isolated VLPs, ATP, CD39 assay buffer, and Colorimetric detection reagent for 96 enzyme reactions. In addition, the kit includes the CD39 inhibitor POM-1 for use as an inhibitor control.
Materials Required But Not Provided:
  • • UV/Vis spectrophotometer microplate reader capable of reading absorbance at 630 nm
  • • Adjustable micropipettor and sterile tips
  • • Rotating or rocker platform (optional)
  • • Aluminum foil 
Synonym(s):
CD39, CD39 VLP, ATP, Virus Like Particle, Ecto-apyrase, ATP Diphosphohydrolase, Ectonucleoside Triphosphate Diphosphohydrolase-1, ENTPD1, vlp, activity assay kit, VLP
Supplied As:
The CD39 VLP assay kit comes in a convenient 96-well format, with full length CD39 enzyme in isolated VLPs, ATP, CD39 assay buffer, and Colorimetric detection reagent for 96 enzyme reactions.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Wang, T.-F., et al., J. Biol. Chem.  273 (38), 24814-21 (1998)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

79583.pdf