CD39 Inhibitor Screening Assay Kit

Catalog #
79278
$890 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The CD39 Inhibitor Screening Assay Kit is a colorimetric assay designed to measure the activity of CD39 for screening and profiling applications. The kit comes in a convenient 96-well format with enough purified CD39 enzyme, ATP, CD39 assay buffer, and colorimetric detection reagent for 100 enzyme reactions. In addition, the kit includes the CD39 inhibitor POM-1 for use as an inhibitor control.

Synonyms
ENTPD1, Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen
Product Info
Storage and Usage
Citations
Assay Kit Format
Colorimetric
Supplied As
The kit comes in a convenient 96-well format with enough purified CD39 enzyme, ATP, CD39 assay buffer, and colorimetric detection reagent for 100 enzyme reactions.
Materials Required But Not Supplied
  • UV/Vis spectrophotometer microplate reader capable of reading λ=630 nm
  • Adjustable micropipettor and sterile tips
Format
Catalog # Reagent Amount Storage
71284 CD39, His-Tag* 1 µg -80°C
79279 4x CD39 Assay Buffer 3 ml -20°C
  35 mM ATP 15 µl -20°C
  POM-1, MW=2986.01 g/mol** 1.5 mg -20°C
74001 Colorimetric Detection Reagent*** 10 ml 4°C
79963 Transparent 96-well microtiter plate 1 Room Temp

 

 

 

 

 

 

 

 

 

 


*The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
**CD39 inhibitor POM-1 is provided as a control for CD39 inhibition.
***Colorimetric Detection Reagent is used to measure the free phosphate generated in the reaction catalyzed by CD39. Any other source of inorganic phosphate will interfere with the assay.

UniProt #
P49961
Background

B cell activation marker Cluster of Differentiation 39 (CD39), also known as ecto-apyrase, ATP diphosphohydrolase, ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1), hydrolyzes extracellular nucleotide tri- and diphosphates into AMP in the presence of Ca2+ and Mg2+. It is an important enzyme in many biological processes, including the modulation of neural cell activities, prevention of intravascular thrombosis, and regulation of immune responses. The role of regulatory T cells (Treg) links closely to CD39/CD79, and a dysfunction in this pathway can lead to cancer progression. The use of CD39 inhibitors can prove advantageous for the treatment of pathologies where CD39/CD73 pathways play a role.

CD73/CD39 Immunotherapy

References

1. Wang, T.-F., et al., J. Biol. Chem. 273 (38), 24814-21 (1998)