CD39 Inhibitor Screening Assay Kit
The CD39 Inhibitor Screening Assay Kit is a colorimetric assay designed to measure the activity of CD39 for screening and profiling applications. The kit comes in a convenient 96-well format with enough purified CD39 enzyme, ATP, CD39 assay buffer, and colorimetric detection reagent for 100 enzyme reactions. In addition, the kit includes the CD39 inhibitor POM-1 for use as an inhibitor control.
- UV/Vis spectrophotometer microplate reader capable of reading λ=630 nm
- Adjustable micropipettor and sterile tips
Catalog # | Reagent | Amount | Storage |
71284 | CD39, His-Tag* | 1 µg | -80°C |
79279 | 4x CD39 Assay Buffer | 3 ml | -20°C |
35 mM ATP | 15 µl | -20°C | |
POM-1, MW=2986.01 g/mol** | 1.5 mg | -20°C | |
74001 | Colorimetric Detection Reagent*** | 10 ml | 4°C |
79963 | Transparent 96-well microtiter plate | 1 | Room Temp |
*The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
**CD39 inhibitor POM-1 is provided as a control for CD39 inhibition.
***Colorimetric Detection Reagent is used to measure the free phosphate generated in the reaction catalyzed by CD39. Any other source of inorganic phosphate will interfere with the assay.
B cell activation marker Cluster of Differentiation 39 (CD39), also known as ecto-apyrase, ATP diphosphohydrolase, ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1), hydrolyzes extracellular nucleotide tri- and diphosphates into AMP in the presence of Ca2+ and Mg2+. It is an important enzyme in many biological processes, including the modulation of neural cell activities, prevention of intravascular thrombosis, and regulation of immune responses. The role of regulatory T cells (Treg) links closely to CD39/CD79, and a dysfunction in this pathway can lead to cancer progression. The use of CD39 inhibitors can prove advantageous for the treatment of pathologies where CD39/CD73 pathways play a role.
1. Wang, T.-F., et al., J. Biol. Chem. 273 (38), 24814-21 (1998)