CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit

Catalog #: 72062
Size: 96 reactions
$1,040
Background:
The activation of naïve T cells requires two signals; the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-2 (CD86) ligand on the APC with the CD28 receptor on the T cell surface. CD28:B7-2 interaction is an important drug target for the regulation of T cells involved in autoimmunity, inflammation, tumor recognition, and immune tolerance.
Description:
The CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-2 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-2 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-2 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Synonym(s):
T-Cell-Specific Surface Glycoprotein, TP44, T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, CD86, B72
Supplied As:
This kit comes in a convenient 96-well format, with biotin-labeled B7-2, purified CD28, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Format:

Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Reference(s):
1. Keir, M.E., et al. Immunol. Rev. 2005, 204: 128-143.

2. Yao, S., et al. Immunity 2011, 34(5):729-40.

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72062.pdf